In this paper, a new Russian sign language multimedia database TheRuSLan is presented. The database includes lexical units (single words and phrases) from Russian sign language within one subject area, namely, “food products at the supermarket”, and was collected using MS Kinect 2.0 device including both FullHD video and the depth map modes, which provides new opportunities for the lexicographical description of the Russian sign language vocabulary and enhances research in the field of automatic gesture recognition. Russian sign language has an official status in Russia, and over 120,000 deaf people in Russia and its neighboring countries use it as their first language. Russian sign language has no writing system, is poorly described and belongs to the low-resource languages. The authors formulate the basic principles of annotation of sign words, based on the collected data, and reveal the content of the collected database. In the future, the database will be expanded and comprise more lexical units. The database is explicitly made for the task of creating an automatic system for Russian sign language recognition.