Chain-of-thought (CoT) advances the reasoning abilities of large language models (LLMs) and achieves superior performance in complex reasoning tasks. However, most CoT studies rely on carefully designed human-annotated rational chains to prompt LLMs, posing challenges for real-world applications where labeled data is available without rational chains. This paper proposes a new strategy, AutomateCoT (Automatic Prompt Augmentation and Selection with Chain-of-Thought), that can bypass human engineering of CoT by automatically augmenting rational chains from a small labeled dataset, and then pruning low-quality chains to construct a candidate pool of machinegenerated rationale chains based on the labels. Finally, it selects the optimal combination of several rationale chains from the pool for CoT prompting by employing a variance-reduced policy gradient strategy to estimate the significance of each example. Automate-CoT enables a quick adaptation of the CoT technique to different tasks. Experimental results demonstrate the effectiveness of our method, where competitive results are achieved on arithmetic reasoning (+2.7%), commonsense reasoning (+3.4%), symbolic reasoning (+3.2%), and non-reasoning tasks (+2.5%).