Large Audio-Language Models (LALMs) integrate audio perception and language understanding within a unified framework, enabling a wide range of real-world applications. Despite recent advances, evaluation for LALMs remains heavily underspecified relative to real-world requirements: most lack true linguistic and cultural authenticity, while others fail to capture acoustic realism. To bridge this gap, we propose GlobeAudio, a multilingual and multicultural benchmark designed to evaluate naturalistic audio understanding. GlobeAudio consists of 5,637 multiple-choice questions across six typologically diverse languages, expertly crafted by native speakers grounded on naturally occurring audio. In order to do well, models must possess higher-level auditory reasoning skills and culturally grounded interpretation. We systematically evaluate representative closed-source and open-source LALMs, as well as cascaded ASR–LLM pipelines. Our experiments reveal substantial performance gaps under natural acoustic conditions, particularly for open-source models and low-resource languages. These findings highlight critical limitations of current LALMs and underscore the importance of naturalistic audio evaluation for future audio-language systems. GlobeAudio can be found at https://huggingface.co/datasets/iNLP-Lab/GlobeAudio.