Africa is home to over 2,000 languages from over six language families and has the highest linguistic diversity among all continents. This includes 75 languages with at least one million speakers each. Yet, there is little NLP research conducted on African languages. Crucial in enabling such research is the availability of high-quality annotated datasets. In this paper, we introduce AfriSenti, a sentiment analysis benchmark that contains a total of >110,000 tweets in 14 African languages (Amharic, Algerian Arabic, Hausa, Igbo, Kinyarwanda, Moroccan Arabic, Mozambican Portuguese, Nigerian Pidgin, Oromo, Swahili, Tigrinya, Twi, Xitsonga, and Yoruba) from four language families. The tweets were annotated by native speakers and used in the AfriSenti-SemEval shared task (with over 200 participants, see website: https://afrisenti-semeval.github.io). We describe the data collection methodology, annotation process, and the challenges we dealt with when curating each dataset. We further report baseline experiments conducted on the AfriSenti datasets and discuss their usefulness.