Existing Graphical User Interface (GUI) reasoning tasks remain challenging, particularly in UI understanding. Current methods typically rely on direct screen-based decision-making, which lacks interpretability and overlooks a comprehensive understanding of UI elements, ultimately leading to task failure. To enhance the understanding and interaction with UIs, we propose an innovative GUI reasoning paradigm called UI-in-the-Loop (UILoop). Our approach treats the GUI reasoning task as a cyclic Screen-UI elements-Action process. By enabling Multimodal Large Language Models (MLLMs) to explicitly learn the localization, semantic functions, and practical usage of key UI elements, UILoop achieves precise element discovery and performs interpretable reasoning. Furthermore, we introduce a more challenging UI Comprehension task centered on UI elements with three evaluation metrics. Correspondingly, we contribute a benchmark of 26K samples (UI Comprehension-Bench) to comprehensively evaluate existing methods’ mastery of UI elements. Extensive experiments demonstrate that UILoop achieves state-of-the-art UI understanding performance while yielding superior results in GUI reasoning tasks.

Real-world professional desktop workflows in specialized creative and engineering software unfold over long horizons and often require human-in-the-loop coordination, where agents proactively seek necessary information and users provide additional instructions, clarifications, feedback, or corrections as the task progresses.Yet existing desktop GUI benchmarks mostly reduce this setting to short, simplified tasks with all user instructions provided upfront.To address this issue, we introduce **DeskCraft**, a desktop GUI benchmark targeting long horizon creative and engineering workflows and proactive human-agent collaboration. DeskCraft organizes tasks into a multilevel difficulty taxonomy, with long horizon tasks requiring over 50 execution steps, and covers professional creative software across design, video, audio, and 3D creation. Furthermore, DeskCraft formalizes human-agent collaboration into an interaction protocol covering *mid-turn* and *post-turn* exchanges. Mid-turn interaction captures both agent-initiated clarification under uncertainty and user-initiated interruption during execution, while post-turn interaction accommodates user-driven feedback after the agent signals completion, together spanning the full space of realistic collaboration patterns. We evaluate 18 proprietary and open source agents on 538 tasks and find that GPT-5.4 reaches **31.6%** on standard tasks and **27.6%** on interactive tasks. Further analyses reveal persistent failures in long horizon workflow delivery and proactive clarification. We will open-source all evaluation codes, tasks, and data.