In this paper we present SenTube – a dataset of user-generated comments on YouTube videos annotated for information content and sentiment polarity. It contains annotations that allow to develop classifiers for several important NLP tasks: (i) sentiment analysis, (ii) text categorization (relatedness of a comment to video and/or product), (iii) spam detection, and (iv) prediction of comment informativeness. The SenTube corpus favors the development of research on indexing and searching YouTube videos exploiting information derived from comments. The corpus will cover several languages: at the moment, we focus on English and Italian, with Spanish and Dutch parts scheduled for the later stages of the project. For all the languages, we collect videos for the same set of products, thus offering possibilities for multi- and cross-lingual experiments. The paper provides annotation guidelines, corpus statistics and annotator agreement details.